Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

Grant, Stephen D. and Cairns, Gemma S. and Wistuba, Jordan and Patton, Brian R. (2019) Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging. F1000Research. ISSN 2046-1402

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    Abstract

    We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the SuperResolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.