Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra
Chung, Li Hung C and Birch, David J S and Vyshemirsky, Vladislav and Bella, Angelo and Ryadnov, Maxim G and Rolinski, Olaf J (2019) Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra. Methods and Applications in Fluorescence, 7 (3). 035003. ISSN 2050-6120 (https://doi.org/10.1088/2050-6120/ab1985)
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Abstract
The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.
ORCID iDs
Chung, Li Hung C ORCID: https://orcid.org/0000-0002-1043-3227, Birch, David J S ORCID: https://orcid.org/0000-0001-6400-1270, Vyshemirsky, Vladislav, Bella, Angelo, Ryadnov, Maxim G and Rolinski, Olaf J ORCID: https://orcid.org/0000-0002-7838-779X;-
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Item type: Article ID code: 68773 Dates: DateEvent16 May 2019Published15 April 2019AcceptedSubjects: Science > Physics Department: Faculty of Science > Physics Depositing user: Pure Administrator Date deposited: 09 Jul 2019 08:11 Last modified: 11 Sep 2024 00:47 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/68773