Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra

Chung, Li Hung C and Birch, David J S and Vyshemirsky, Vladislav and Bella, Angelo and Ryadnov, Maxim G and Rolinski, Olaf J (2019) Protein fibrillogenesis model tracked by its intrinsic time-resolved emission spectra. Methods and Applications in Fluorescence, 7 (3). 035003. ISSN 2050-6120

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    Abstract

    The excited-state kinetics of the fluorescence of tyrosine in a de novo protein fibrillogenesis model was investigated as a potential tool for monitoring protein fibre formation and complexation with glucose (glycation). In stark contrast to insulin the time-resolved emission spectra (TRES) recorded over the period of 700 hours in buffered solutions of the model with and without glucose revealed no apparent changes in Tyr fluorescence responses. This indicates the stability of the model and provides a measurement-supported basis for its use as a reference material in fluorescence studies of protein aggregation.