Picture of DNA strand

Pioneering chemical biology & medicinal chemistry through Open Access research...

Strathprints makes available scholarly Open Access content by researchers in the Department of Pure & Applied Chemistry, based within the Faculty of Science.

Research here spans a wide range of topics from analytical chemistry to materials science, and from biological chemistry to theoretical chemistry. The specific work in chemical biology and medicinal chemistry, as an example, encompasses pioneering techniques in synthesis, bioinformatics, nucleic acid chemistry, amino acid chemistry, heterocyclic chemistry, biophysical chemistry and NMR spectroscopy.

Explore the Open Access research of the Department of Pure & Applied Chemistry. Or explore all of Strathclyde's Open Access research...

Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

Kataropoulou, M. and Henderson, C.J. and Grant, M.H. (2002) Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition. Toxicology, 192. pp. 87-88. ISSN 0300-483X

Full text not available in this repository.Request a copy from the Strathclyde author

Abstract

The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.