New insights into the molecular effects and probiotic properties of Lactobacillus pentosus pre-adapted to edible oils
Alonso García, Esther and Pérez Montoro, Beatriz and Benomar, Nabil and Castillo-Gutiérrez, Sonia and Estudillo-Martínez, María D. and Knapp, Charles W. and Abriouel, Hikmate (2019) New insights into the molecular effects and probiotic properties of Lactobacillus pentosus pre-adapted to edible oils. Food Science and Technology, 109. pp. 153-162. ISSN 0023-6438 (https://doi.org/10.1016/j.lwt.2019.04.028)
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Abstract
In this study, the survival and growth of seven probiotic Lactobacillus pentosus strains isolated from Aloreña green table olives in the presence of vegetable-based edible oils (i.e., sunflower, olive, linseed, soy, corn, almond and argan) and mint essential oil were determined for the first time. Slight decreases in bacterial viability were observed depending on the strain and oil exposure, mainly mint essential oil. However, pre-adaptating the strains to the corresponding oils significantly increased their cell viabilities. As such, this study examined whether pre-adaptating probiotic L. pentosus strains with oils will constitute a new strategy to increase stress resistance, e.g., acids (pH 1.5) or bile (up to 3.6%) in food production and/or during digestion, and improve functional probiotic properties. Improvements in stress resistance (acidic and bile conditions) were noticed in some pre-adapted strains with oils; further, pre-adaptations with olive, argan, sunflower and linseed oils induced gene expression (e.g., fus, rpsL, pgm, groEL, enol and prep) for moonlighting proteins involved in several stress responses and other functions. As such, pre-adaptation with vegetable edible oils may represent a novel approach for manufacturing probiotic products by improving the stability of bacteria during industrial processes that would otherwise reduce their viability and functionality.
ORCID iDs
Alonso García, Esther, Pérez Montoro, Beatriz, Benomar, Nabil, Castillo-Gutiérrez, Sonia, Estudillo-Martínez, María D., Knapp, Charles W. ORCID: https://orcid.org/0000-0001-7997-8543 and Abriouel, Hikmate;-
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Item type: Article ID code: 67562 Dates: DateEvent31 July 2019Published10 April 2019Published Online8 April 2019Accepted25 October 2018SubmittedSubjects: Technology > Engineering (General). Civil engineering (General) > Environmental engineering Department: Faculty of Engineering > Civil and Environmental Engineering Depositing user: Pure Administrator Date deposited: 12 Apr 2019 09:49 Last modified: 28 Sep 2024 13:58 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/67562