Single-molecule localization microscopy analysis with ImageJ
van de Linde, Sebastian (2019) Single-molecule localization microscopy analysis with ImageJ. Journal of Physics D: Applied Physics, 52 (20). 203002. ISSN 1361-6463 (https://doi.org/10.1088/1361-6463/ab092f)
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Abstract
ImageJ is a versatile and powerful tool for quantitative image analysis in microscopy. It is open-source software, platform-independent and enables students and researchers to obtain an easy but thorough introduction into image analysis. Especially the image processing package Fiji is a valuable and powerful extension of ImageJ. Several plugins and macros for single-molecule localization microscopy (SMLM) have been developed during the last decade. These novel tools cover the steps from single-molecule localization and image reconstruction to SMLM data postprocessing such as density analysis, image registration or resolution estimation. This article describes how ImageJ/Fiji can be used for image analysis, reviews existing extensions for SMLM, and aims at introducing and motivating novices and advanced SMLM users alike to explore the possibilities of ImageJ/Fiji for automated and quantitative data analysis.
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Item type: Article ID code: 67360 Dates: DateEvent13 March 2019Published21 February 2019Published Online21 February 2019Accepted1 October 2018SubmittedSubjects: Science > Physics Department: Faculty of Science > Physics Depositing user: Pure Administrator Date deposited: 19 Mar 2019 12:40 Last modified: 16 Oct 2024 00:33 URI: https://strathprints.strath.ac.uk/id/eprint/67360