Effects of silk degumming process on physicochemical, tensile, and optical properties of regenerated silk fibroin

Nultsch, Kira and Bast, Livia K. and Näf, Muriel and Yakhlifi, Salima El and Bruns, Nico and Germershaus, Oliver (2018) Effects of silk degumming process on physicochemical, tensile, and optical properties of regenerated silk fibroin. Macromolecular Materials and Engineering, 303 (12). 1800408. ISSN 1439-2054

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    Abstract

    Sericin removal from silk (degumming) affects material characteristics of silk fibroin (SF). Sodium carbonate is most commonly used for degumming, but numerous alternative methods are available. Herein, a systematic comparison of degumming methods is provided. Sodium carbonate, sodium oleate, trypsin, and ionic liquid are used, and materials are characterized regarding mass loss, SF content, molecular integrity of SF, refractive index, and tensile properties. Complete degumming is achieved within 30 min of using sodium carbonate, but results in significant reduction of molecular weight, shift toward less acidic charge variants, and reduction of yield- and rupture force. Sodium oleate and trypsin are inefficient and negatively affect tensile properties, while ionic liquid shows good efficiency and marginal degradation of SF but also reduced yield- and rupture force. Refractive index is not affected by degumming. These results allow rational selection of the degumming method and tuning of SF properties for biomedical applications.