Cre-dependent optogenetic transgenic mice without early age-related hearing loss
Lyngholm, Daniel and Sakata, Shuzo (2019) Cre-dependent optogenetic transgenic mice without early age-related hearing loss. Frontiers in Aging Neuroscience, 11. 29. ISSN 1663-4365 (https://doi.org/10.3389/fnagi.2019.00029)
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Abstract
With the advent of recent genetic technologies for mice, it is now feasible to investigate the circuit mechanisms of brain functions in an unprecedented manner. Although transgenic mice are commonly used on C57BL/6J (C57) background, hearing research has typically relied on different genetic backgrounds, such as CBA/Ca or CBA due to the genetic defect of C57 mice for early age-related hearing loss. This limits the utilization of available genetic resources for hearing research. Here we report congenic (>F10) Cre-dependent channelrhodopsin2 (ChR2) mice on CBA/Ca background. By crossing this line with Cre-driver mice on C57 background, F1 hybrids restored the hearing deficit of C57 mice. We also found a linear relationship between aging and hearing loss, with progression rates varied depending on genetic backgrounds (3.39 dB/month for C57; 0.82 dB/month for F1 hybrid). We further demonstrate that this approach allows to express ChR2 in a specific type of inhibitory neurons in the auditory cortex and that they can be identified within a simultaneously recorded population of neurons in awake mice. Thus, our Cre-dependent optogenetic transgenic mice on CBA/Ca background are a valuable tool to investigate the circuit mechanisms of hearing across lifespan.
ORCID iDs
Lyngholm, Daniel ORCID: https://orcid.org/0000-0002-3708-0249 and Sakata, Shuzo ORCID: https://orcid.org/0000-0001-6796-411X;-
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Item type: Article ID code: 66868 Dates: DateEvent26 February 2019Published1 February 2019AcceptedSubjects: Medicine > Internal medicine > Neuroscience. Biological psychiatry. Neuropsychiatry
Medicine > Pharmacy and materia medicaDepartment: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 07 Feb 2019 14:48 Last modified: 05 Sep 2024 00:59 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/66868