Chaperonin–dendrimer conjugates for siRNA delivery

Nussbaumer, Martin G. and Duskey, Jason T. and Rother, Martin and Renggli, Kasper and Chami, Mohamed and Bruns, Nico (2016) Chaperonin–dendrimer conjugates for siRNA delivery. Advanced Science, 3 (10). 1600046. ISSN 2198-3844

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    Abstract

    The group II chaperonin thermosome (THS) is a hollow protein nanoparticle that can encapsulate macromolecular guests. Two large pores grant access to the interior of the protein cage. Poly(amidoamine) (PAMAM) is conjugated into THS to act as an anchor for small interfering RNA (siRNA), allowing to load the THS with therapeutic payload. THS–PAMAM protects siRNA from degradation by RNase A and traffics KIF11 and GAPDH siRNA into U87 cancer cells. By modification of the protein cage with the cell-penetrating peptide TAT, RNA interference is also induced in PC-3 cells. THS–PAMAM protein–polymer conjugates are therefore promising siRNA transfection reagents and greatly expand the scope of protein cages in drug delivery applications.