Surface enhanced Raman scattering (SERS) based microfluidics for single cell analysis
Willner, Marjorie R. and McMillan, Kay and Graham, Duncan and Vikesland, Peter J. and Zagnoni, Michele (2018) Surface enhanced Raman scattering (SERS) based microfluidics for single cell analysis. Analytical Chemistry. ISSN 0003-2700 (https://doi.org/10.1021/acs.analchem.8b02636)
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Abstract
The integration of surface enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin (WGA) functionalized SERS nanoprobes in water-in-oil droplets that were subsequently locked into a storage droplet array for spectroscopic investigation. The stationary droplets enabled the rapid identification of SERS regions of interest in live cancer cells by allowing collection of “fast” coarse maps over an area of several mm2 followed by “slower” detailed interrogation of the identified hotspots. We demonstrate SERS at cellular resolution via a proof-of-concept assay that detects glycan expression on the surface of prostate cancer cells using WGA modified metallic nanoparticles. The data illustrates the potential of SERS optofluidic systems for high-throughput cell screening and illustrates a previously unobserved high degree of cell-to-cell variability in the size and number of glycan islands.
ORCID iDs
Willner, Marjorie R., McMillan, Kay ORCID: https://orcid.org/0000-0001-8710-2496, Graham, Duncan ORCID: https://orcid.org/0000-0002-6079-2105, Vikesland, Peter J. and Zagnoni, Michele ORCID: https://orcid.org/0000-0003-3198-9491;-
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Item type: Article ID code: 65543 Dates: DateEvent19 September 2018Published19 September 2018Published Online19 September 2018AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Pure and Applied Chemistry
Faculty of Engineering > Electronic and Electrical EngineeringDepositing user: Pure Administrator Date deposited: 26 Sep 2018 10:20 Last modified: 08 Oct 2024 03:42 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/65543