An empirical analysis of enzyme function reporting for experimental reproducibility : missing/incomplete information in published papers
Halling, Peter and Fitzpatrick, Paul F. and Raushel, Frank M. and Rohwer, Johann and Schnell, Santiago and Wittig, Ulrike and Wohlgemuth, Roland and Kettner, Carsten (2018) An empirical analysis of enzyme function reporting for experimental reproducibility : missing/incomplete information in published papers. Biophysical Chemistry, 242. pp. 22-27. ISSN 0301-4622 (https://doi.org/10.1016/j.bpc.2018.08.004)
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Abstract
A key component of enzyme function experiments is reporting of considerable metadata, to allow other researchers to replicate, interpret properly or use fully the results. This paper evaluates the completeness of enzyme function data reporting for reproducibility. We present a detailed examination of 11 recent papers (and their supplementary material) from two leading journals. We found that in every paper we were not able to collect some critical information necessary to reproduce the enzyme function findings. Study of 100 papers used by the SABIO-RK database confirmed some of the most common omissions: concentration of enzyme or its substrates, identity of counter-ions in buffers. A computer system should be better at preventing such omissions, helping secure the scientific record. Many of the omissions found would be trapped by the currently available version of STRENDA DB.
ORCID iDs
Halling, Peter ORCID: https://orcid.org/0000-0001-5077-4088, Fitzpatrick, Paul F., Raushel, Frank M., Rohwer, Johann, Schnell, Santiago, Wittig, Ulrike, Wohlgemuth, Roland and Kettner, Carsten;-
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Item type: Article ID code: 65415 Dates: DateEvent30 November 2018Published24 August 2018Published Online20 August 2018AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 13 Sep 2018 14:03 Last modified: 10 Oct 2024 03:01 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/65415