Standing wave microscopy of red blood cell membrane morphology with high temporal resolution

Tinning, Peter W. and Scrimgeour, Ross and McConnell, Gail (2018) Standing wave microscopy of red blood cell membrane morphology with high temporal resolution. In: ELMI 2018, 2018-06-05 - 2018-06-08.

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    Abstract

    Widefield fluorescence microscopy is an integral tool for life science imaging though the achievable resolutions are limited by the diffraction nature of light. One technique to increase the axial resolution is known as standing wave microscopy [1]. The standing wave can be generated by placing a mirror at the specimen plane which causes interference between the incoming and reflected excitation illumination. The axial resolution is reduced to λ/4n as only fluorophores which are in the location of the full width at the half maximum of the antinodes are excited [2] resulting in periodic bands of fluorescence.