The serine proteinase hepsin is an activator of pro-matrix metalloproteinases : molecular mechanisms and implications for extracellular matrix turnover
Wilkinson, David J. and Desilets, Antoine and Lin, Hua and Charlton, Sarah and Del Carmen Arques, Maria and Falconer, Adrian and Bullock, Craig and Hsu, Yu Chen and Birchall, Kristian and Hawkins, Alastair and Thompson, Paul and Ferrell, William R. and Lockhart, John and Plevin, Robin and Zhang, Yadan and Blain, Emma and Lin, Shu Wha and Leduc, Richard and Milner, Jennifer M. and Rowan, Andrew D. (2017) The serine proteinase hepsin is an activator of pro-matrix metalloproteinases : molecular mechanisms and implications for extracellular matrix turnover. Scientific Reports, 7 (1). 16693. ISSN 2045-2322 (https://doi.org/10.1038/s41598-017-17028-3)
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Abstract
Increasing evidence implicates serine proteinases in the proteolytic cascades leading to the pathological destruction of extracellular matrices such as cartilage in osteoarthritis (OA). We have previously demonstrated that the type II transmembrane serine proteinase (TTSP) matriptase acts as a novel initiator of cartilage destruction via the induction and activation of matrix metalloproteinases (MMPs). Hepsin is another TTSP expressed in OA cartilage such that we hypothesized this proteinase may also contribute to matrix turnover. Herein, we demonstrate that addition of hepsin to OA cartilage in explant culture induced significant collagen and aggrecan release and activated proMMP-1 and proMMP-3. Furthermore, hepsin directly cleaved the aggrecan core protein at a novel cleavage site within the interglobular domain. Hepsin expression correlated with synovitis as well as tumour necrosis factor α expression, and was induced in cartilage by a pro-inflammatory stimulus. However, a major difference compared to matriptase was that hepsin demonstrated markedly reduced capacity to activate proteinase-activated receptor-2. Overall, our data suggest that hepsin, like matriptase, induces potent destruction of the extracellular matrix whilst displaying distinct efficiencies for the cleavage of specific substrates.
ORCID iDs
Wilkinson, David J., Desilets, Antoine, Lin, Hua, Charlton, Sarah, Del Carmen Arques, Maria, Falconer, Adrian, Bullock, Craig, Hsu, Yu Chen, Birchall, Kristian, Hawkins, Alastair, Thompson, Paul, Ferrell, William R., Lockhart, John, Plevin, Robin ORCID: https://orcid.org/0000-0002-7849-1220, Zhang, Yadan, Blain, Emma, Lin, Shu Wha, Leduc, Richard, Milner, Jennifer M. and Rowan, Andrew D.;-
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Item type: Article ID code: 62761 Dates: DateEvent1 December 2017Published21 November 2017AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
University of Strathclyde > University of StrathclydeDepositing user: Pure Administrator Date deposited: 09 Jan 2018 11:02 Last modified: 30 Aug 2024 00:55 URI: https://strathprints.strath.ac.uk/id/eprint/62761