Toxoplasma modulates signature pathways of human epilepsy, neurodegeneration & cancer

Ngô, Huân M. and Zhou, Ying and Lorenzi, Hernan and Wang, Kai and Kim, Taek-Kyun and Zhou, Yong and Bissati, Kamal El and Mui, Ernest and Fraczek, Laura and Rajagopala, Seesandra V. and Roberts, Craig W. and Montpetit, Alexandre and Blackwell, Jenefer M. and Jamieson, Sarra E. and Wheeler, Kelsey and Begeman, Ian J. and Naranjo-Galvis, Carlos and Alliey-Rodriguez, Ney and Davis, Roderick G. and Soroceanu, Liliana and Cobbs, Charles and Steindler, Dennis A. and Boyer, Kenneth and Noble, A. Gwendolyn and Swisher, Charles N. and Heydemann, Peter T. and Rabiah, Peter and Withers, Shawn and Soteropoulos, Patricia and Hood, Leroy and McLeod, Rima (2017) Toxoplasma modulates signature pathways of human epilepsy, neurodegeneration & cancer. Scientific Reports, 7 (1). 11496. ISSN 2045-2322

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    One third of humans are infected lifelong with the brain-dwelling, protozoan parasite, Toxoplasma gondii. Approximately fifteen million of these have congenital toxoplasmosis. Although neurobehavioral disease is associated with seropositivity, causality is unproven. To better understand what this parasite does to human brains, we performed a comprehensive systems analysis of the infected brain: We identified susceptibility genes for congenital toxoplasmosis in our cohort of infected humans and found these genes are expressed in human brain. Transcriptomic and quantitative proteomic analyses of infected human, primary, neuronal stem and monocytic cells revealed effects on neurodevelopment and plasticity in neural, immune, and endocrine networks. These findings were supported by identification of protein and miRNA biomarkers in sera of ill children reflecting brain damage and T. gondii infection. These data were deconvoluted using three systems biology approaches: "Orbital-deconvolution" elucidated upstream, regulatory pathways interconnecting human susceptibility genes, biomarkers, proteomes, and transcriptomes. "Cluster-deconvolution" revealed visual protein-protein interaction clusters involved in processes affecting brain functions and circuitry, including lipid metabolism, leukocyte migration and olfaction. Finally, "disease-deconvolution" identified associations between the parasite-brain interactions and epilepsy, movement disorders, Alzheimer's disease, and cancer. This "reconstruction-deconvolution" logic provides templates of progenitor cells' potentiating effects, and components affecting human brain parasitism and diseases.

    ORCID iDs

    Ngô, Huân M., Zhou, Ying, Lorenzi, Hernan, Wang, Kai, Kim, Taek-Kyun, Zhou, Yong, Bissati, Kamal El, Mui, Ernest, Fraczek, Laura, Rajagopala, Seesandra V., Roberts, Craig W. ORCID logoORCID:, Montpetit, Alexandre, Blackwell, Jenefer M., Jamieson, Sarra E., Wheeler, Kelsey, Begeman, Ian J., Naranjo-Galvis, Carlos, Alliey-Rodriguez, Ney, Davis, Roderick G., Soroceanu, Liliana, Cobbs, Charles, Steindler, Dennis A., Boyer, Kenneth, Noble, A. Gwendolyn, Swisher, Charles N., Heydemann, Peter T., Rabiah, Peter, Withers, Shawn, Soteropoulos, Patricia, Hood, Leroy and McLeod, Rima;