Picture of smart phone

Open Access research that is better understanding human-computer interaction...

Strathprints makes available scholarly Open Access content by researchers in the Department of Computer & Information Sciences, including those researching information retrieval, information behaviour, user behaviour and ubiquitous computing.

The Department of Computer & Information Sciences hosts The Mobiquitous Lab, which investigates user behaviour on mobile devices and emerging ubiquitous computing paradigms. The Strathclyde iSchool Research Group specialises in understanding how people search for information and explores interactive search tools that support their information seeking and retrieval tasks, this also includes research into information behaviour and engagement.

Explore the Open Access research of The Mobiquitous Lab and the iSchool, or theDepartment of Computer & Information Sciences more generally. Or explore all of Strathclyde's Open Access research...

Rapid fabrication of cell-laden alginate hydrogel 3D structures by micro dip-coating

Ghanizadeh Tabriz, Atabak and Mills, Christopher G. and Mullins, John J. and Davies, James A. and Shu, Wenmiao (2017) Rapid fabrication of cell-laden alginate hydrogel 3D structures by micro dip-coating. Frontiers in Bioengineering and Biotechnology, 5 (13). ISSN 2296-4185

[img]
Preview
Text (Ghanizadeh-Tabriz-etal-FBB2017-Rapid-fabrication-of-cell-laden-alginate-hydrogel-3D-structures)
Ghanizadeh_Tabriz_etal_FBB2017_Rapid_fabrication_of_cell_laden_alginate_hydrogel_3D_structures.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (5MB) | Preview

Abstract

Development of a simple, straight-forward 3D fabrication method to culture cells in 3D, without relying on any complex fabrication methods, remains a challenge. In this paper, we describe a new technique that allows fabrication of scalable 3D cell-laden hydrogel structures easily, without complex machinery: the technique can be done using only apparatus already available in a typical cell biology laboratory. The fabrication method involves micro dip-coating of cell-laden hydrogels covering the surface of a metal bar, into the cross-linking reagents calcium chloride or barium chloride to form hollow tubular structures. This method can be used to form single layers with thickness ranging from 126 μm to 220 μm or multi-layered tubular structures. This fabrication method uses alginate hydrogel as the primary biomaterial and a secondary biomaterial can be added depending on the desired application. We demonstrate the feasibility of this method, with survival rate over 75% immediately after fabrication and normal responsiveness of cells within these tubular structures using mouse dermal embryonic fibroblast cells and human embryonic kidney 293 cells containing a tetracycline-responsive, red fluorescent protein (tHEK cells).