Part II : influence of dimerization of a modified GnRH-I peptide sequence on a male antifertility vaccine

Ferro, Valerie A. and Harvey, Michael J. A. and Colston, Angela and Stimson, William H. (2002) Part II : influence of dimerization of a modified GnRH-I peptide sequence on a male antifertility vaccine. American Journal of Reproductive Immunology, 48 (6). pp. 372-380. ISSN 1600-0897

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Abstract

PROBLEM: In the previous paper, we described how the tetanus toxoid (TT) conjugated monomer, CHWSYGLRPG-NH2, induced high neutralizing antibody titres, which resulted in decreased levels of testosterone and subsequent antifertility. However, its counterpart HWSYGLRPGC, induced low avidity antibody titres. We wanted to know whether peptide dimerization would improve the efficacy of both peptides. METHOD OF STUDY: Male Sprague-Dawley rats were immunized with modified dimerized GnRH-I peptides (HWSYGLRPGCCGPRLGYSWH and GPRLGYSWHCCHWSYGLRPG-NH2), with or without conjugation to TT. RESULTS: The unconjugated dimers were not effective in causing castration, although the first peptide dimer did induce production of antibodies. When conjugated to TT, both dimers showed the same level of efficacy in causing castration as each other. However, there were differences in antibody binding to native GnRH. CONCLUSIONS: Dimerization and conjugation to a carrier improved the antifertility efficacy of HWSYGLRPGC, whereas the conjugated monomer CHWSYGLRPG-NH2 showed a greater level of consistent castration than its conjugated dimer.

Author(s):Ferro, Valerie A., Harvey, Michael J. A., Colston, Angela and Stimson, William H.
Item type:Article
ID code:59612
Keywords:amino acid sequence, animals, antibody affinity, antibody specificity, atrophy, dimerization, enzyme-linked immunosorbent assay, follicle stimulating hormone, gonadotropin-releasing hormone, hormone antagonists, immunization, immunoglobulin G, immunoglobulin M, luteinizing hormone, male, molecular sequence data, oligopeptides, organ size, peptide fragments, rats, rats, sprague-dawley, spermatogenesis, structure-activity relationship, testis, testosterone, vaccines, contraceptive, Immunology, Immunology, Obstetrics and Gynaecology, Reproductive Medicine
Subjects:Science > Microbiology > Immunology
Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date deposited:26 Jan 2017 16:27
Last modified:03 Jan 2019 15:00
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URI:https://strathprints.strath.ac.uk/id/eprint/59612
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