Investigation into suitable carrier molecules for use in an anti-gonadotrophin releasing hormone vaccine
Ferro, V. A. and Stimson, W. H. (1998) Investigation into suitable carrier molecules for use in an anti-gonadotrophin releasing hormone vaccine. Vaccine, 16 (11-12). pp. 1095-1102. ISSN 0264-410X
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Gonadal function can be controlled through immunoneutralisation of gonadotrophin releasing hormone (GnRH), with an analogue, GnRH-glycys, linked to a carrier molecule and an appropriate adjuvant. In this study, four different types of carrier molecule were investigated: (a) single and branched amino acid polymers--[poly-(D-glu, D-lys) and poly-(phe, glu)-poly(DL-ala)-poly(lys)]; (b) bacterial toxoids--diphtheria (DT) and tetanus (TT); (c) synthetic T-helper epitopes--derived from malarial circumsporozite protein (CS) and measles virus fusion protein (MVF); and (d) thyroglobulin (Thy)--a large protein. The effect of non-ionic surfactant vesicles (NISV) and an aluminum hydroxide based adjuvant (alum), was also examined. Although good antibody responses were achieved with GnRH-glycys-DT, GnRH-glycys-TT and GnRH-glycys-Thy, adsorbed onto alum and the dimerised synthetic T-helper epitope constructs, incorporated into NISV, a critical antibody titre was necessary to result in morphological changes in the gonads and complete suppression of spermatogenesis. This was only achieved with tetanus toxoid and the dimerised T-helper epitopes.
Creators(s): |
Ferro, V. A. ![]() | Item type: | Article |
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ID code: | 59609 |
Keywords: | immunogenicity, carrier molecules , GnRH analogue, gonadal function, Immunology, Infectious Diseases, Molecular Medicine, Public Health, Environmental and Occupational Health, veterinary(all), Immunology and Microbiology(all) |
Subjects: | Science > Microbiology > Immunology |
Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
Depositing user: | Pure Administrator |
Date deposited: | 26 Jan 2017 15:00 |
Last modified: | 21 Jan 2021 00:43 |
Related URLs: | |
URI: | https://strathprints.strath.ac.uk/id/eprint/59609 |
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