Biofluids and other techniques : general discussion

Goodacre, Royston and Baker, Matthew J. and Graham, Duncan and Schultz, Zachary D. and Diem, Max and Marques, Maria Paula and Cinque, Gianfelice and Vernooij, Robbin and Sulé-Suso, Josep and Byrne, Hugh J. and Faulds, Karen and Hermes, Michael and Fleming, Holly and Bonifacio, Alois and Dluhy, Richard and Gardner, Peter and El-Mashtoly, Samir and Wood, Bayden and Gough, Kathleen and Fornasaro, Stefano and Kazarian, Sergei and Jamieson, Lauren and Petrich, Wolfgang and Sockalingum, Ganesh Dhruvananda and Stone, Nick and Kendall, Catherine and Sinjab, Faris and Haris, Parvez and Subaihi, Abdu and Remiszewski, Stan and Hellwig, Petra and Sergo, Valter and Gerwert, Klaus and Phillips, Christopher and Campbell, Colin J. (2016) Biofluids and other techniques : general discussion. Faraday Discussions, 187. pp. 575-601. ISSN 1359-6640

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Abstract

Richard Dluhy opened a general discussion of the paper by Duncan Graham: In your example of a heterogeneous solution-based assay for multicomponent analysis, what is the concentration of the target fungal ssPCR DNA that is used, and how do you manage the kinetics of the reaction such that the target reaches the probe in a time frame appropriate for a clinical assay?