Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution
Wei, Guoke and Yu, Jun and Wang, Jinliang and Gu, Peng and Birch, David J.S. and Chen, Yu (2016) Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution. Journal of Biomedical Optics, 21 (9). 097001. ISSN 1083-3668 (https://doi.org/10.1117/1.JBO.21.9.097001)
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Abstract
We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.
ORCID iDs
Wei, Guoke, Yu, Jun ORCID: https://orcid.org/0000-0002-3673-6760, Wang, Jinliang, Gu, Peng, Birch, David J.S. ORCID: https://orcid.org/0000-0001-6400-1270 and Chen, Yu;-
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Item type: Article ID code: 57443 Dates: DateEvent8 September 2016Published16 August 2016AcceptedNotes: Wei, G., Yu, J., Wang, J., Gu, P., Birch, D. J. S., & Chen, Y. Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution. Journal of Biomedical Optics, JBO 160218RR (2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Science > PhysicsDepartment: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Physics
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 17 Aug 2016 13:43 Last modified: 03 Oct 2024 00:25 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/57443