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Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy

Bonnier, Franck and Brachet, Guillaume and Duong, Romain and Sojinrin, Tobiloba and Respaud, Renaud and Aubrey, Nicolas and Baker, Matthew J. and Byrne, Hugh J. and Chourpa, Igor (2016) Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy. Journal of Biophotonics. pp. 1-16. ISSN 1864-063X

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Abstract

Vibrational spectroscopic techniques can detect small variations in molecular content, linked with disease, showing promise for screening and early diagnosis. Biological fluids, particularly blood serum, are potentially valuable for diagnosis purposes. The so-called Low Molecular Weight Fraction (LMWF) contains the associated peptidome and metabolome and has been identified as potentially the most relevant molecular population for disease-associated biomarker research. Although vibrational spectroscopy can deliver a specific chemical fingerprint of the samples, the High Molecular Weight Fraction (HMWF), composed of the most abundant serum proteins, strongly dominates the response and ultimately makes the detection of minor spectral variations a challenging task. Spectroscopic detection of potential serum biomarkers present at relatively low concentrations can be improved using pre-analytical depletion of the HMWF. In the present study, human serum fractionation by centrifugal filtration was used prior to analysis by Attenuated Total Reflection infrared spectroscopy. Using a model sample based on glycine spiked serum, it is demonstrated that the screening of the LMWF can be applied to quantify blinded concentrations up to 50 times lower. Moreover, the approach is easily transferable to different bodily fluids which would support the development of more efficient and suitable clinical protocols exploring vibrational spectroscopy based ex-vivo diagnostic tools.

Item type: Article
ID code: 57410
Notes: This is the peer reviewed version of the following article: Bonnier, F., Brachet, G., Duong, R., Sojinrin, T., Respaud, R., Aubrey, N., ... Chourpa, I. (2016). Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy. Journal of Biophotonics, 1-16, which has been published in final form at 10.1002/jbio.201600015. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Keywords: human serum, centrifugal filtration, IR spectroscopy, attenuated total reflection, principal component analysis, low molecular weight fraction, Optics. Light, Biology, Atomic and Molecular Physics, and Optics, Applied Microbiology and Biotechnology
Subjects: Science > Physics > Optics. Light
Science > Natural history > Biology
Department: Faculty of Science > Pure and Applied Chemistry
Depositing user: Pure Administrator
Date deposited: 15 Aug 2016 10:15
Last modified: 21 Sep 2017 13:43
URI: https://strathprints.strath.ac.uk/id/eprint/57410
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