Picture map of Europe with pins indicating European capital cities

Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

Explore research outputs by the European Policies Research Centre...

Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy

Bonnier, Franck and Brachet, Guillaume and Duong, Romain and Sojinrin, Tobiloba and Respaud, Renaud and Aubrey, Nicolas and Baker, Matthew J. and Byrne, Hugh J. and Chourpa, Igor (2016) Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy. Journal of Biophotonics. pp. 1-16. ISSN 1864-063X

[img]
Preview
Text (Bonnier-etal-JB-2016-screening-the-low-molecular-weight-fraction-of-human-serum)
Bonnier_etal_JB_2016_screening_the_low_molecular_weight_fraction_of_human_serum.pdf - Accepted Author Manuscript

Download (1MB) | Preview

Abstract

Vibrational spectroscopic techniques can detect small variations in molecular content, linked with disease, showing promise for screening and early diagnosis. Biological fluids, particularly blood serum, are potentially valuable for diagnosis purposes. The so-called Low Molecular Weight Fraction (LMWF) contains the associated peptidome and metabolome and has been identified as potentially the most relevant molecular population for disease-associated biomarker research. Although vibrational spectroscopy can deliver a specific chemical fingerprint of the samples, the High Molecular Weight Fraction (HMWF), composed of the most abundant serum proteins, strongly dominates the response and ultimately makes the detection of minor spectral variations a challenging task. Spectroscopic detection of potential serum biomarkers present at relatively low concentrations can be improved using pre-analytical depletion of the HMWF. In the present study, human serum fractionation by centrifugal filtration was used prior to analysis by Attenuated Total Reflection infrared spectroscopy. Using a model sample based on glycine spiked serum, it is demonstrated that the screening of the LMWF can be applied to quantify blinded concentrations up to 50 times lower. Moreover, the approach is easily transferable to different bodily fluids which would support the development of more efficient and suitable clinical protocols exploring vibrational spectroscopy based ex-vivo diagnostic tools.