Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection
O'Reilly, Emmet J. and Conroy, Paul J. and Hearty, Stephen and Keyes, Tia E. and O'Kennedy, Richard and Forster, Robert J. and Dennany, Lynn (2015) Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection. RSC Advances, 5 (83). pp. 67874-67877. ISSN 2046-2069 (https://doi.org/10.1039/C5RA08450D)
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Abstract
This work exploits the high-affinity of recombinant antibodies and low background electrochemiluminescence (ECL) for cardiac-biomarker detection. The developed assay is capable of fg mL-1 detection limits as well as the detection of C-Reactive Protein (CRP) over a clinically relevant range. The assay demonstrated robust reproducibility, selectivity and stability while also highlighting a novel platform for detection of cardiac biomarkers at low concentrations.
ORCID iDs
O'Reilly, Emmet J., Conroy, Paul J., Hearty, Stephen, Keyes, Tia E., O'Kennedy, Richard, Forster, Robert J. and Dennany, Lynn ORCID: https://orcid.org/0000-0002-5481-1066;-
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Item type: Article ID code: 55990 Dates: DateEvent31 July 2015Published31 July 2015AcceptedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 23 Mar 2016 14:30 Last modified: 06 Sep 2024 00:43 URI: https://strathprints.strath.ac.uk/id/eprint/55990