Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents
Zhao, Jinlian and Evangelopoulos, Dimitrios and Bhakta, Sanjib and Gray, Alexander I. and Seidel, Veronique (2014) Antitubercular activity of Arctium lappa and Tussilago farfara extracts and constituents. Journal of Ethnopharmacology, 155 (1). pp. 796-800. ISSN 1872-7573 (https://doi.org/10.1016/j.jep.2014.06.034)
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Arctium lappa and Tussilago farfara (Asteraceae) are two plant species used traditionally as antitubercular remedies. The aim of this study was (i) to screen Arctium lappa and Tussilago farfara extracts for activity against Mycobacterium tuberculosis and (ii) to isolate and identify the compound(s) responsible for this reputed anti-TB effect. The activity of extracts and isolated compounds was determined against Mycobacterium tuberculosis H37Rv using a high throughput spot culture growth inhibition (HT-SPOTi) assay. The n-hexane extracts of both plants, the ethyl acetate extract of Tussilago farfara and the dichloromethane phase derived from the methanol extract of Arctium lappa displayed antitubercular activity (MIC 62.5 μg/mL). Further chemical investigation of Arctium lappa led to the isolation of n-nonacosane (1), taraxasterol acetate (2), taraxasterol (3), a (1:1) mixture of β sitosterol/stigmasterol (4), isololiolide (5), melitensin (6), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10). Compounds isolated from Tussilago farfara were identified as a (1:1) mixture of β sitosterol/stigmasterol (4), trans-caffeic acid (7), kaempferol (8), quercetin (9), kaempferol-3-O-glucoside (10), loliolide (11), a (4:1) mixture of p-coumaric acid/4-hydroxybenzoic acid (12), p-coumaric acid (13). All compounds were identified following analyses of their physicochemical and spectroscopic data (MS, 1H and 13C-NMR) and by comparison with published data. This is the first report of the isolation of n-nonacosane (1), isololiolide (5), melitensin (6) and kaempferol-3-O-glucoside (10) from Arctium lappa, and of loliolide (11) from Tussilago farfara. Amongst the isolated compounds, the best activity was observed for p-coumaric acid (13) (MIC 31.3 μg/mL or 190.9 μM) alone and in mixture with 4-hydroxybenzoic acid (12) (MIC 62.5 μg/mL). The above results provide for the first time some scientific evidence to support, to some extent, the ethno-medicinal use of Arctium lappa and Tussilago farfara as traditional antitubercular remedies.
ORCID iDs
Zhao, Jinlian, Evangelopoulos, Dimitrios, Bhakta, Sanjib, Gray, Alexander I. and Seidel, Veronique ORCID: https://orcid.org/0000-0003-3880-5261;-
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Item type: Article ID code: 54070 Dates: DateEvent8 August 2014Published20 June 2014Published Online14 June 2014AcceptedSubjects: Medicine > Pharmacy and materia medica
Medicine > Therapeutics. PharmacologyDepartment: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 25 Aug 2015 15:00 Last modified: 11 Nov 2024 10:45 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/54070