Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

McMillan, Kay and Boyd, Marie and Zagnoni, Michele; (2014) Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids. In: MicroTAS 2014. CBM Society, USA, pp. 923-925. ISBN 9780979806476 (http://www.rsc.org/images/loc/2014/PDFs/Papers/303...)

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Abstract

In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

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