Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids
McMillan, Kay and Boyd, Marie and Zagnoni, Michele (2014) Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids. In: MicroTAS 2014. CBM Society, San Diego, CA., pp. 923-925. ISBN 9780979806476
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In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.
| Author(s): | McMillan, Kay, Boyd, Marie and Zagnoni, Michele | Item type: | Book Section |
|---|---|
| ID code: | 53028 |
| Keywords: | cancer, spheroids, radiotherapy, droplet microfluidics, 3D cell culture, Neoplasms. Tumors. Oncology (including Cancer), Biochemistry, medical, Oncology |
| Subjects: | Medicine > Internal medicine > Neoplasms. Tumors. Oncology (including Cancer) |
| Department: | Faculty of Engineering > Electronic and Electrical Engineering Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Technology and Innovation Centre > Advanced Science and Technology Technology and Innovation Centre > Bionanotechnology |
| Depositing user: | Pure Administrator |
| Date deposited: | 15 May 2015 10:24 |
| Last modified: | 30 Jan 2019 10:25 |
| Related URLs: | |
| URI: | https://strathprints.strath.ac.uk/id/eprint/53028 |
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