Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

McMillan, Kay and Boyd, Marie and Zagnoni, Michele; (2014) Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids. In: MicroTAS 2014. CBM Society, USA, pp. 923-925. ISBN 9780979806476

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Abstract

In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

ORCID iDs

McMillan, Kay ORCID logoORCID: https://orcid.org/0000-0001-8710-2496, Boyd, Marie ORCID logoORCID: https://orcid.org/0000-0003-4120-2218 and Zagnoni, Michele ORCID logoORCID: https://orcid.org/0000-0003-3198-9491;
CORE (COnnecting REpositories)