Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids

McMillan, Kay and Boyd, Marie and Zagnoni, Michele (2014) Development of a droplet microfluidic assay for radiotherapy treatment of multicellular spheroids. In: MicroTAS 2014. CBM Society, USA, pp. 923-925. ISBN 9780979806476

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Abstract

In this study, we report the development of a droplet microfluidic assay for the formation and radiotherapy treatment of multicellular spheroids. Glioma cells formed multicellular spheroids following their encapsulation within biocompatible medium-in-fluorinated oil (M/O) droplets with a non-adherent surfactant interface. Our studies identified that the ratio of volume-of-medium to number-of-cells and the refreshment of medium are important parameters for healthy spheroid growth within M/O droplets. In addition, a protocol was validated to extract radiotherapy dose responses from the spheroids based on their size variation via brightfield microscopy.

Author(s):	McMillan, Kay ORCID: https://orcid.org/0000-0001-8710-2496, Boyd, Marie ORCID: https://orcid.org/0000-0003-4120-2218 and Zagnoni, Michele ORCID: https://orcid.org/0000-0003-3198-9491
Item type:	Book Section
ID code:	53028
Keywords:	cancer, spheroids, radiotherapy, droplet microfluidics, 3D cell culture, Neoplasms. Tumors. Oncology (including Cancer), Biochemistry, medical, Oncology
Subjects:	Medicine > Internal medicine > Neoplasms. Tumors. Oncology (including Cancer)
Department:	Faculty of Engineering > Electronic and Electrical Engineering Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Technology and Innovation Centre > Advanced Science and Technology Technology and Innovation Centre > Bionanotechnology
Depositing user:	Pure Administrator
Date deposited:	15 May 2015 10:24
Last modified:	08 Jan 2020 17:17
Related URLs:	Event
URI:	https://strathprints.strath.ac.uk/id/eprint/53028
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