Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source
McConnell, G. and Girkin, J.M. and Poland, S. (2006) Beyond monochromatic light: three-dimensional confocal laser scanning microscopy using a supercontinuum source. Proceedings of SPIE: The International Society for Optical Engineering, 6047. 2Q1 - 2Q5. (http://www.10.1117/12.710991)
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Confocal laser scanning microscopy (CLSM) has rapidly become an essential tool in the life sciences laboratory, enabling high-resolution and minimally intrusive optical sectioning of fluorescent samples. Most commercially available CLSM systems employ a gas laser, e.g. a Kr/Ar laser, to provide the excitation radiation. However, such lasers have several shortcomings, including the maintenance requirements, short lifetimes and high noise levels. To overcome these limitations, a light source for CLSM that is based on supercontinuum generation in photonic crystal fiber has been developed. This source provides the necessary wavelength range required to excite the widest possible variety of fluorophores. A novel method of extracting the desired wavelengths from the supercontinuum source using a digital micro-mirror device (DMD) is also described.
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Item type: Article ID code: 5300 Dates: DateEvent27 October 2006PublishedNotes: Proceedings title: Fourth International Conference on Photonics and Imaging in Biology and Medicine. Subjects: Science > Physics > Optics. Light Department: Faculty of Science > Physics > Institute of Photonics
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical SciencesDepositing user: Strathprints Administrator Date deposited: 03 Feb 2008 Last modified: 08 Apr 2024 16:06 URI: https://strathprints.strath.ac.uk/id/eprint/5300