Microneedle/nanoencapsulation-mediated transdermal delivery : mechanistic insights
Gomaa, Yasmine A. and Garland, Martin J. and McInnes, Fiona J. and Donnelly, Ryan F. and El-Khordagui, Labiba K. and Wilson, Clive G. (2014) Microneedle/nanoencapsulation-mediated transdermal delivery : mechanistic insights. European Journal of Pharmaceutics and Biopharmaceutics, 86 (2). pp. 145-155. ISSN 0939-6411 (https://doi.org/10.1016/j.ejpb.2013.01.026)
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Abstract
A systematic study was undertaken to gain more insight into the mechanism of transdermal delivery of nanoencapsulated model dyes across microneedle (MN)-treated skin, a complex process not yet explored. Rhodamine B (Rh B) and fluorescein isothiocyanate (FITC) as model hydrophilic and hydrophobic small/medium-size molecules, respectively, were encapsulated in poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) and delivered through full thickness porcine skin pretreated with MN array. Permeation through MN-treated skin was affected by physicochemical characteristics of NPs and the encapsulated dyes. Dye flux was enhanced by smaller particle size, hydrophilicity, and negative zeta potential of NPs. Regarding encapsulated dyes, solubility at physiological pH and potential interaction with skin proteins proved to outweigh molecular weight as determinants of skin permeation. Data were verified using confocal laser scanning microscopy imaging. Findings coupled with the literature data are supportive of a mechanism involving influx of NPs, particularly of smaller size, deep into MN-created channels, generating depot dye-rich reservoirs. Molecular diffusion of the released dye across viable skin layers proceeds at a rate determined by its molecular characteristics. Data obtained provide mechanistic information of importance to the development of formulation strategies for more effective intradermal and transdermal MN-mediated delivery of nanoencapsulated therapeutic agents.
ORCID iDs
Gomaa, Yasmine A., Garland, Martin J., McInnes, Fiona J., Donnelly, Ryan F., El-Khordagui, Labiba K. and Wilson, Clive G. ORCID: https://orcid.org/0000-0002-4211-7907;-
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Item type: Article ID code: 49033 Dates: DateEventFebruary 2014Published24 February 2013Published OnlineSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 12 Aug 2014 06:47 Last modified: 01 Oct 2024 00:21 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/49033