Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Mackenzie, Ashleigh and Stewart, Lewis and Hoskisson, Paul A and Tucker, Nicholas P; Filloux, Alain and Ramos, Juan-Luis, eds. (2014) Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. In: Pseudomonas Methods and Protocols. Methods in Molecular Biology . Springer, New York, pp. 607-612. ISBN 9781493904723 (https://doi.org/10.1007/978-1-4939-0473-0_46)

Abstract

Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.

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• Item metadata

  Item type:	Book Section
  ID code:	48124
  Dates:	Date Event 2014 Published
  Subjects:	Medicine > Therapeutics. Pharmacology Medicine > Pharmacy and materia medica Science > Microbiology
  Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
  Depositing user:	Pure Administrator
  Date deposited:	19 May 2014 09:26
  Last modified:	08 Apr 2024 13:11
  URI:	https://strathprints.strath.ac.uk/id/eprint/48124