Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Mackenzie, Ashleigh and Stewart, Lewis and Hoskisson, Paul A and Tucker, Nicholas P (2014) Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. In: Pseudomonas Methods and Protocols. Methods in Molecular Biology . Springer, New York, pp. 607-612. ISBN 9781493904723

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Abstract

Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.