Spermine-induced hybridization and charge inversion at the diffuse layer of a DNA-FET
De La Rica, Roberto and Fernandez-Sanchez, Cesar and Cecilia, Jimenez-Jorquera and Baldi, Antonio (2008) Spermine-induced hybridization and charge inversion at the diffuse layer of a DNA-FET. Journal of Physical Chemistry B, 112 (25). pp. 7614-7617. ISSN 1520-6106 (https://doi.org/10.1021/jp711348m)
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Detection of DNA hybridization events by using field-effect transducers is limited by the electrolyte content of the medium. So far, DNA was thought to hybridize only in solutions with concentrated electrolytes. In these media, the interface between the transducer gate and the solution is reduced to a thin layer in close contact with the surface, and DNA is poorly detected. In the present work, this limitation is overcome by using spermine as screening polycation. Hybridization assays with polycation concentrations as low as 10 mu M are reported. This ensures that hybridization takes place at the diffuse layer of the interface. The reported results suggest a charge-inversion mechanism induced by spermine. A target sequence is real-time label-free detected in the range from 10 to 500 nM.
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Item type: Article ID code: 45762 Dates: DateEvent26 June 2008Published31 May 2008Published OnlineSubjects: Science > Physics Department: Faculty of Science > Physics Depositing user: Pure Administrator Date deposited: 08 Nov 2013 10:07 Last modified: 08 Apr 2024 20:55 URI: https://strathprints.strath.ac.uk/id/eprint/45762