Supramolecular Au nanoparticle assemblies as optical probes for enzyme-linked immunoassays

De La Rica, Roberto and Velders, Aldrik (2011) Supramolecular Au nanoparticle assemblies as optical probes for enzyme-linked immunoassays. Small, 7 (1). 66–69. ISSN 1613-6810

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Abstract

A novel enzyme-linked immunoassay with nanoparticle assemblies as optical probes is presented. The macrocycle cucurbit[7]uril assembles gold nanoparticles, and the enzymatic generation of ammonium by urease as the label of the immunoassay disperses the probes to yield variations of plasmon absorbance that are proportional to the target protein concentration.