Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye

De La Rica, Roberto and Stevens, Molly M. (2012) Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye. Nature Nanotechnology, 7 (12). 821–824. ISSN 1748-3387 (https://doi.org/10.1038/nnano.2012.186)

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Abstract

In resource-constrained countries, affordable methodologies for the detection of disease biomarkers at ultralow concentrations can potentially improve the standard of living1, 2. However, current strategies for ultrasensitive detection often require sophisticated instruments that may not be available in laboratories with fewer resources3, 4, 5, 6, 7, 8, 9, 10, 11. Here, we circumvent this problem by introducing a signal generation mechanism for biosensing that enables the detection of a few molecules of analyte with the naked eye. The enzyme label of an enzyme-linked immunosorbent assay (ELISA) controls the growth of gold nanoparticles and generates coloured solutions with distinct tonality when the analyte is present. Prostate specific antigen (PSA) and HIV-1 capsid antigen p24 were detected in whole serum at the ultralow concentration of 1 × 10−18 g ml−1. p24 was also detected with the naked eye in the sera of HIV-infected patients showing viral loads undetectable by a gold standard nucleic acid-based test

  • Item type:Article
    ID code:45683
    Dates:
    Date
    Event
    December 2012
    Published
    Subjects:Science > Physics
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:06 Nov 2013 10:31
    Last modified:19 Apr 2024 09:15
    URI:https://strathprints.strath.ac.uk/id/eprint/45683
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