Protein coated microcrystals formulated with model antigens and modified with calcium phosphate exhibit enhanced phagocytosis and immunogenicity
Jones, Sarah and Asokanathan, Catpagavalli and Kmiec, Dorota and Irvine, June and Fleck, Roland and Xing, Dorothy and Moore, Barry and Parton, Roger and Coote, John (2014) Protein coated microcrystals formulated with model antigens and modified with calcium phosphate exhibit enhanced phagocytosis and immunogenicity. Vaccine, 32 (33). pp. 4234-4242. ISSN 1873-2518 (https://doi.org/10.1016/j.vaccine.2013.09.061)
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Abstract
Protein-coated microcrystals (PCMCs) were investigated as potential vaccine formulations for a range of model antigens. Presentation of antigens as PCMCs increased the antigen-specific IgG responses for all antigens tested, compared to soluble antigens. When compared to conventional aluminium-adjuvanted formulations, PCMCs modified with calcium phosphate (CaP) showed enhanced antigen-specific IgG responses and a decreased antigen-specific IgG1:IgG2a ratio, indicating the induction of a more balanced Th1/Th2 response. The rate of antigen release from CaP PCMCs, in vitro, decreased strongly with increasing CaP loading but their immunogenicity in vivo was not significantly different, suggesting the adjuvanticity was not due to a depot effect. Notably, it was found that CaP modification enhanced the phagocytosis of fluorescent antigen-PCMC particles by J774.2 murine monocyte/macrophage cells compared to soluble antigen or soluble PCMCs. Thus, CaP PCMCs may provide an alternative to conventional aluminium-based acellular vaccines to provide a more balanced Th1/Th2 immune response.
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Item type: Article ID code: 45574 Dates: DateEvent16 July 2014Published10 October 2013Published Online26 September 2013AcceptedSubjects: Medicine > Pharmacy and materia medica
Science > ChemistryDepartment: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 04 Nov 2013 11:38 Last modified: 08 Aug 2024 01:11 URI: https://strathprints.strath.ac.uk/id/eprint/45574