Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays

Singh, Ishwar and Wendeln, Christian and Clark, Alasdair W. and Cooper, Jonathan M. and Ravoo, Bart Jan and Burley, Glenn A. (2013) Sequence-selective detection of double-stranded DNA sequences using pyrrole-imidazole polyamide microarrays. Journal of the American Chemical Society, 135 (9). pp. 3449-3457. ISSN 0002-7863

[img]
Preview
Text (Singh-etal-JACS2013-Sequence-selective-detection-of-double-stranded-DNA)
Singh_etal_JACS2013_Sequence_selective_detection_of_double_stranded_DNA.pdf
Final Published Version
License: Creative Commons Attribution 4.0 logo

Download (8MB)| Preview

    Abstract

    We describe a microarray format that can detect double-stranded DNA sequences with a high degree of sequence selectivity. Cyclooctyne-derivatized pyrrole-imidazole polyamides were immobilized on azide-modified glass substrates using microcontact printing and a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. These polyamide-immobilized substrates selectively detected a seven-base-pair binding site incorporated within a double-stranded oligodeoxyribonucleotide sequence even in the presence of an excess of a sequence with a single-base-pair mismatch