The helminth product, ES-62, protects against airway inflammation by resetting the Th cell phenotype
Rzepecka, Justyna and Siebeke, Ivonne and Coltherd, Jennifer and Kean, Dorothy and Steiger, Christina and Al-Riyami, Lamyaa and McSharry, Charles and Harnett, Margaret and Harnett, William (2013) The helminth product, ES-62, protects against airway inflammation by resetting the Th cell phenotype. International Journal for Parasitology, 43 (3-4). 211–223. ISSN 0020-7519 (https://doi.org/10.1016/j.ijpara.2012.12.001)
PDF.
Filename: PARA_3496.pdf
Preprint Download (2MB) |
Abstract
We previously demonstrated inhibition of ovalbumin (OVA)-induced allergic airway hyper-responsiveness in the mouse using ES-62, a phosphorylcholine-containing glycoprotein secreted by the filarial nematode, Acanthocheilonema viteae. This inhibition correlated with ES-62-induced mast cell desensitisation, although the degree to which this reflected direct targeting of mast cells remained unclear as suppression of the Th2 phenotype of the inflammatory response, as measured by eosinophilia and IL-4 levels in the lungs, was also observed. We now show that inhibition of the lung Th2 phenotype is reflected in ex vivo analyses of draining lymph node recall cultures and accompanied by a decrease in the serum levels of total and OVA-specific IgE. Moreover, ES-62 also suppresses the lung infiltration by neutrophils that is associated with severe asthma and is generally refractory to conventional anti-inflammatory therapies, including steroids. Protection against Th2-associated airway inflammation does not reflect induction of regulatory T cell (Treg) responses (there is no increased IL-10 or Foxp3 expression) but rather a switch in polarisation towards increased T-bet expression and IFNγ production. This ES-62-driven switch in the Th1/Th2 balance is accompanied by decreased IL-17 responses, a finding in line with reports that IFNγ and IL-17 are counter-regulatory. Consistent with ES-62 mediating its effects via IFNγ-mediated suppression of pathogenic Th2/Th17 responses, we found that neutralising anti-IFNγ antibodies blocked protection against airway inflammation in terms of pro-inflammatory cell infiltration, particularly by neutrophils and lung pathology. Collectively, these studies indicate that ES-62, or more likely small molecule analogues, could have therapeutic potential in asthma, in particular for those subtypes of patients (e.g. smokers, steroid-resistant) who are refractory to current treatments.
ORCID iDs
Rzepecka, Justyna, Siebeke, Ivonne, Coltherd, Jennifer, Kean, Dorothy, Steiger, Christina, Al-Riyami, Lamyaa, McSharry, Charles, Harnett, Margaret and Harnett, William ORCID: https://orcid.org/0000-0001-9545-9401;-
-
Item type: Article ID code: 42507 Dates: DateEventMarch 2013PublishedSubjects: Medicine > Therapeutics. Pharmacology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 09 Jan 2013 14:30 Last modified: 07 Oct 2024 00:15 URI: https://strathprints.strath.ac.uk/id/eprint/42507