Urine metabolomics reveals novel physiologic functions of human aldehyde oxidase and provides biomarkers for typing xanthinuria

Peretz, Hava and Watson, David G. and Blackburn, Gavin and Zhang, Tong and Lagziel, Ayala and Shtauber-Naamati, Meirav and Morad, Tova and Keren-Tardai, Elena and Greenshpun, Victoria and Usher, Sali and Shalev, Hanna and Landau, Daniel and Levartovsky, David (2012) Urine metabolomics reveals novel physiologic functions of human aldehyde oxidase and provides biomarkers for typing xanthinuria. Metabolomics, 8 (5). pp. 951-959. ISSN 1573-3882 (https://doi.org/10.1007/s11306-011-0391-3)

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Classical xanthinuria is a rare inherited metabolic disorder caused by either isolated xanthine dehydrogenase (XDH) deficiency (type I) or combined XDH and aldehyde oxidase (AO) deficiency (type II). XDH and AO are evolutionary related enzymes that share a sulfurated molybdopterin cofactor. While the role of XDH in purine metabolism is well established, the physiologic functions of AO are mostly unknown. XDH and AO are important drug metabolizing enzymes. Urine metabolomic analysis by high pressure liquid chromatography and mass spectrometry of xanthinuric patients was performed to unveil physiologic functions of XDH and AO and provide biomarkers for typing xanthinuria. Novel endogenous products of AO, hydantoin propionic acid, N1-methyl-8-oxoguanine and N-(3-acetamidopropyl) pyrrolidin-2-one formed in the histidine, nucleic acid and spermidine metabolic pathways, respectively, were identified as being lowered in type II xanthinuria. Also lowered were the known AO products, N1-methyl-2-pyridone-5-carboxamide and N1-methyl-4-pyridone-5-carboxamide in the nicotinamide degradation pathway. In contrast to the KEGG annotations, the results suggest minor role of human AO in the conversion of pyridoxal to pyridoxate and gentisaldehyde to gentisate in the vitamin B6 and tyrosine metabolic pathways, respectively. The perturbations in purine degradation due to XDH deficiency radiated further from the previously known metabolites, uric acid, xanthine and hypoxanthine to guanine, methyl guanine, xanthosine and inosine. Possible pathophysiological implications of the observed metabolic perturbations are discussed. The identified biomarkers have the potential to replace the allopurinol-loading test used in the past to type xanthinuria, thus facilitating appropriate pharmacogenetic counseling and gene directed search for causative mutations.


Peretz, Hava, Watson, David G. ORCID logoORCID: https://orcid.org/0000-0003-1094-7604, Blackburn, Gavin, Zhang, Tong, Lagziel, Ayala, Shtauber-Naamati, Meirav, Morad, Tova, Keren-Tardai, Elena, Greenshpun, Victoria, Usher, Sali, Shalev, Hanna, Landau, Daniel and Levartovsky, David;