Cancellous bone allograft seeded with human mesenchymal stromal cells : a potential good manufacturing practice-grade tool for the regeneration of bone defects

Stiehler, Maik and Seib, F Philipp and Rauh, Juliane and Goedecke, Anja and Werner, Carsten and Bornhäuser, Martin and Günther, Klaus-Peter and Bernstein, Peter (2010) Cancellous bone allograft seeded with human mesenchymal stromal cells : a potential good manufacturing practice-grade tool for the regeneration of bone defects. Cytotherapy, 12 (5). pp. 658-668.

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Abstract

Combining autologous bone precursor cells with cancellous bone allograft (CBA) offers an appealing strategy for skeletal regeneration. In this context, multipotent mesenchymal stromal cells (MSC) provide an excellent cell source because they are readily harvested from donors, expanded and differentiated in vitro. The aim of this study was to evaluate the proliferation, morphology, osteogenic differentiation and stem cell-related gene expression during static long-term ex vivo cultivation using human MSC and CBA under good manufacturing practice (GMP)-conforming conditions.