Specificity of the volume-activated amino acid efflux pathway in cultured human breast cancer cells
Shennan, D. B. and Thomson, J. (2011) Specificity of the volume-activated amino acid efflux pathway in cultured human breast cancer cells. General Physiology and Biophysics, 30 (1). pp. 45-51. ISSN 0231-5882 (https://doi.org/10.4149/gpb_2011_01_45)
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It has been shown that cell swelling stimulates the efflux of taurine from MCF-7 and MDA-MB-231 cells via a pathway which has channel-like properties. The purpose of this study was to examine the specificity of the volume-activated taurine efflux pathway in both cell lines. A hyposmotic shock increased the efflux of glycine, L-alanine, AIB (α-aminoisobutyric acid), D-aspartate but not L-leucine from MDA-MB-231 and MCF-7 cells. It was evident that the time course of activation/inactivation of those amino acids whose efflux was affected by cell swelling was similar to that of volume-activated taurine efflux. The effect of exogenous ATP on swelling-induced glycine, AIB and D-aspartate efflux from MDA-MB-231 cells was similar to that found on taurine efflux. In addition, volume-activated AIB efflux from MDA-MB-231 cells, like that of swelling-induced taurine efflux, was inhibited by diiodosalicylate. Tamoxifen inhibited volume-activated taurine release from both MDA-MB-231 and MCF-7 cells. The results suggest that neutral and anionic α-amino acids are able to utilize the volume-activated taurine efflux pathway in both cell lines. The effect of tamoxifen on breast cancer growth may, in part, be related to perturbations in cell volume regulation.
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Item type: Article ID code: 40576 Dates: DateEvent2011PublishedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 26 Jul 2012 10:48 Last modified: 08 Apr 2024 20:02 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/40576