Light-induced Ca(2+) transients observed in widefield epi-fluorescence microscopy of excitable cells
McDonald, Alison and Harris, John and MacMillan, Debbi and Dempster, John and McConnell, Gail (2012) Light-induced Ca(2+) transients observed in widefield epi-fluorescence microscopy of excitable cells. Biomedical Optics Express, 3 (6). pp. 1266-1273. (https://doi.org/10.1364/BOE.3.001266)
Full text not available in this repository.Request a copyAbstract
We have investigated the possibility that variations in the level of intracellular Ca(2+) in excitable cells might be induced as an artifact of the incoherent illumination that is being used to monitor transient responses. In order to avoid the fluctuations in power of an arc lamp source, a microscope using a light emitting diode that was calibrated accurately at low power levels, was constructed to provide good control over the dose of light applied to the biological specimen. We report here that higher powers of illumination increased the probability of occurrence of Ca(2+) transients even in the sub-mW range normally used to measure such transients in epi-fluorescence work, suggesting that caution should be exercised when designing experiments and interpreting data
-
-
Item type: Article ID code: 40250 Dates: DateEvent2012Published4 May 2012Published OnlineSubjects: Medicine > Therapeutics. Pharmacology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 29 Jun 2012 09:25 Last modified: 08 Apr 2024 19:59 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/40250