Sphingosine kinase 1 is an intracellular effector of phosphatidic acid

Delon, C. and Manifava, M. and Wood, E. and Thompson, D. and Krugmann, S. and Pyne, S. and Ktistakis, N.T. (2004) Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. Journal of Biological Chemistry, 279. pp. 44763-44774. ISSN 1083-351X

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Abstract

Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. The SK1-PA interaction maps to the C terminus of SK1 and is independent of catalytic activity or of the diacylglycerol kinase-like domain of the enzyme. Thus SK1 constitutes a novel, physiologically relevant PA effector.

Creators(s): Delon, C., Manifava, M., Wood, E., Thompson, D., Krugmann, S., Pyne, S. ORCID logoORCID: https://orcid.org/0000-0002-6608-9584 and Ktistakis, N.T.;
Item type:Article
ID code:37938
Keywords:sphingosine kinase 1, intracellular effector , phosphatidic acid, Pharmacy and materia medica, Biochemistry, Cell Biology, Molecular Biology
Subjects:Medicine > Pharmacy and materia medica
Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and Pharmacology
Depositing user: Pure Administrator
Date deposited:27 Feb 2012 14:40
Last modified:23 Oct 2020 04:16
URI:https://strathprints.strath.ac.uk/id/eprint/37938
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