Sphingosine kinase 1 is an intracellular effector of phosphatidic acid
Delon, C. and Manifava, M. and Wood, E. and Thompson, D. and Krugmann, S. and Pyne, S. and Ktistakis, N.T. (2004) Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. Journal of Biological Chemistry, 279. pp. 44763-44774. ISSN 1083-351X (https://doi.org/10.1074/jbc.M405771200)
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Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. The SK1-PA interaction maps to the C terminus of SK1 and is independent of catalytic activity or of the diacylglycerol kinase-like domain of the enzyme. Thus SK1 constitutes a novel, physiologically relevant PA effector.
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Item type: Article ID code: 37938 Dates: DateEvent2004PublishedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Physiology and PharmacologyDepositing user: Pure Administrator Date deposited: 27 Feb 2012 14:40 Last modified: 09 May 2024 04:33 URI: https://strathprints.strath.ac.uk/id/eprint/37938