Experimental systems for studying the role of G-protein-coupled receptors in receptor tyrosine kinase signal transduction

Pyne, Nigel J and Waters, Catherine and Moughal, Noreen Akhtar and Sambi, Balwinder and Connell, Michelle and Pyne, Susan (2004) Experimental systems for studying the role of G-protein-coupled receptors in receptor tyrosine kinase signal transduction. Methods in Enzmology, 390. pp. 451-475. ISSN 0076-6879

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Abstract

Early conception of G-protein-coupled receptor (GPCR) and receptor tyrosine kinase (RTK) signaling pathways was that each represented distinct and linear modules that converged on downstream targets, such as p42/p44 mitogen-activated protein kinase (MAPK). It has now become clear that this is not the case and that multiple levels of cross-talk exist between both receptor systems at early points during signaling events. In recent years, it has become apparent that transactivation of receptor tyrosine kinases by GPCR agonists is a general phenomenon that has been demonstrated for many unrelated GPCRs and receptor tyrosine kinases. In this case, GPCR/G-protein participation is upstream of the receptor tyrosine kinase. However, evidence now demonstrates that numerous growth factors use G proteins and associated signaling molecules such as beta-arrestins that participate downstream of the receptor tyrosine kinase to signal to effectors, such as p42/p44 MAPK. This article highlights experimental approaches used to investigate this novel mechanism of cross-talk between receptor tyrosine kinases and GPCRs.

Creators(s): Pyne, Nigel J ORCID logoORCID: https://orcid.org/0000-0002-5657-4578, Waters, Catherine, Moughal, Noreen Akhtar, Sambi, Balwinder, Connell, Michelle and Pyne, Susan ORCID logoORCID: https://orcid.org/0000-0002-6608-9584;
Item type:Article
ID code:34969
Keywords:animals, arrestins, cell line, humans, mitogen-activated protein kinases, pertussis toxin, phosphorylation, receptor protein-tyrosine Kinases, receptor, nerve growth factor, receptor, platelet-derived Growth Factor beta, receptors, G-protein-coupled, receptors, lysosphingolipid, signal transduction, tyrosine, tyrphostins, Pharmacy and materia medica, Biochemistry, Molecular Biology
Subjects:Medicine > Pharmacy and materia medica
Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date deposited:11 Nov 2011 16:53
Last modified:01 Jan 2021 10:04
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URI:https://strathprints.strath.ac.uk/id/eprint/34969
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