Attomolar detection of protein biomarkers using biofunctionalized gold nanorods with surface plasmon resonance
Sim, H. R. and Wark, Alastair and Lee, H. J. (2010) Attomolar detection of protein biomarkers using biofunctionalized gold nanorods with surface plasmon resonance. Analyst, 135 (10). pp. 2528-2532. ISSN 0003-2654 (https://doi.org/10.1039/c0an00457j)
Preview |
PDF.
Filename: Analyst_2010_Wark_final.pdf
Accepted Author Manuscript Download (821kB)| Preview |
Abstract
This paper describes an ultrasensitive surface plasmon resonance (SPR) detection method using biofunctionalized gold nanorods for the direct detection of protein biomarkers. Immunoglobulin E (IgE), which has separate antibody and DNA aptamer binding sites, was chosen as a model protein for which a sandwich assay platform was designed. Detection was achieved via the specific adsorption of unlabelled IgE proteins onto the surface immobilized aptamer followed by the specific adsorption of anti-IgE coated gold nanorods (Au-NRs). Using the biofunctionalized nanorods in conjunction with SPR, we were able to directly measure IgE proteins at attomolar concentrations. This is a remarkable 108 enhancement compared to conventional SPR measurements of the same surface sandwich assay format ‘anti-IgE/IgE/surface bound IgE-aptamer’ in the absence of gold nanorod signal amplification.
ORCID iDs
Sim, H. R., Wark, Alastair ORCID: https://orcid.org/0000-0001-8736-7566 and Lee, H. J.;-
-
Item type: Article ID code: 34119 Dates: DateEvent20 August 2010PublishedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Pure Administrator Date deposited: 20 Oct 2011 12:50 Last modified: 03 Oct 2024 00:20 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/34119