Picture of neon light reading 'Open'

Discover open research at Strathprints as part of International Open Access Week!

23-29 October 2017 is International Open Access Week. The Strathprints institutional repository is a digital archive of Open Access research outputs, all produced by University of Strathclyde researchers.

Explore recent world leading Open Access research content this Open Access Week from across Strathclyde's many research active faculties: Engineering, Science, Humanities, Arts & Social Sciences and Strathclyde Business School.

Explore all Strathclyde Open Access research outputs...

Strathprints

Deletion of UDP-glucose pyrophosphorylase reveals a UDP-glucose independent UDP-galactose salvage pathway in Leishmania major

Lamerz, Anne-Christin and Damerow, Sebastian and Kleczka, Barbara and Wiese, Martin and van Zandbergen, Ger and Lamerz, Jens and Wenzel, Alexander and Hsu, Fong-Fu and Turk, John and Beverley, Stephen M and Routier, Françoise H (2010) Deletion of UDP-glucose pyrophosphorylase reveals a UDP-glucose independent UDP-galactose salvage pathway in Leishmania major. Glycobiology, 20 (7). pp. 872-882.

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

The nucleotide sugar UDP-galactose (UDP-Gal) is essential for the biosynthesis of several abundant glycoconjugates forming the surface glycocalyx of the protozoan parasite Leishmania major. Current data suggest that UDP-Gal could arise de novo by epimerization of UDP-glucose (UDP-Glc) or by a salvage pathway involving phosphorylation of Gal and the action of UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase as described by Leloir. Since both pathways require UDP-Glc, inactivation of the UDP-glucose pyrophosphorylase (UGP) catalyzing activation of glucose-1 phosphate to UDP-Glc was expected to deprive parasites of UDP-Gal required for Leishmania glycocalyx formation. Targeted deletion of the gene encoding UGP, however, only partially affected the synthesis of the Gal-rich phosphoglycans. Moreover, no alteration in the abundant Gal-containing glycoinositolphospholipids was found in the deletion mutant. Consistent with these findings, the virulence of the UGP-deficient mutant was only modestly affected. These data suggest that Leishmania elaborates a UDP-Glc independent salvage pathway for UDP-Gal biosynthesis.

Item type: Article
ID code: 33843
Keywords: amino acid sequence, animals, humans, leishmania major, macrophages, mice, mice, inbred BALB C, molecular sequence data, sequence deletion, signal transduction, UTP-glucose-1-phosphate uridylyltransferase, uridine diphosphate galactose, uridine diphosphate glucose, Pharmacy and materia medica, Biochemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date deposited: 11 Oct 2011 14:52
Last modified: 14 Jul 2017 03:41
Related URLs:
URI: https://strathprints.strath.ac.uk/id/eprint/33843
Export data:
CORE (COnnecting REpositories)