The potential of mass spectrometry for the global profiling of parasite metabolomes

Watson, D G (2010) The potential of mass spectrometry for the global profiling of parasite metabolomes. Parasitology, 137 (9). pp. 1409-1423.

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Official URL: https://doi.org/10.1017/S0031182009991648

Abstract

The strengths and limitations of existing mass spectrometry methods for metabolite detection and identification are discussed. A brief review is made of the methods available for quenching and extraction of cells or organisms prior to instrumental analysis. The techniques available for carrying out mass spectrometry-based profiling of metabolomes are discussed using the analysis of extracts from trypanosomes to illustrate various points regarding methods of separation and mass spectrometric analysis. The advantages of hydrophilic interaction chromatography (HILIC) for the analysis of polar metabolites are discussed. The challenges of data processing are outlined and illustrated using the example of ThermoFisher's Sieve software. The existing literature on applications of mass spectrometry to the profiling of parasite metabolomes is reviewed.

Author(s):	Watson, D G ORCID: https://orcid.org/0000-0003-1094-7604
Item type:	Article
ID code:	33839
Keywords:	animals, mass spectrometry, metabolome, metabolomics, parasites, parasitology, trypanosomes, orbitrap, hydrophilic interaction chromatography , extraction, GC-MS, global profiling, Pharmacy and materia medica, Infectious Diseases, Animal Science and Zoology, Parasitology
Subjects:	Medicine > Pharmacy and materia medica
Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user:	Pure Administrator
Date deposited:	11 Oct 2011 14:24
Last modified:	22 Jan 2020 06:47
Related URLs:	Scopus publication
URI:	https://strathprints.strath.ac.uk/id/eprint/33839
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