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In vivo real-time multiphoton imaging of T lymphocytes in the mouse brain after experimental stroke

Fumagalli, Stefano and Coles, Jonathan A and Ejlerskov, Patrick and Ortolano, Fabrizio and Bushell, Trevor J and Brewer, James M and De Simoni, Maria-Grazia and Dever, Gary and Garside, Paul and Maffia, Pasquale and Carswell, Hilary V (2011) In vivo real-time multiphoton imaging of T lymphocytes in the mouse brain after experimental stroke. Stroke, 42 (5). pp. 1429-1436. ISSN 0039-2499

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Abstract

BACKGROUND AND PURPOSE: To gain a better understanding of T cell behavior after stroke, we have developed real-time in vivo brain imaging of T cells by multiphoton microscopy after middle cerebral artery occlusion. METHODS: Adult male hCD2-GFP transgenic mice that exhibit green fluorescent protein-labeled T cells underwent permanent left distal middle cerebral artery occlusion by electrocoagulation (n=6) or sham surgery (n=6) and then multiphoton laser imaging 72 hours later. RESULTS: Extravasated T cell number significantly increased after middle cerebral artery occlusion versus sham. Two T cell populations existed after middle cerebral artery occlusion, possibly driven by 2 T cell subpopulations; 1 had significantly lower and the other significantly higher track velocity and displacement rate than sham. CONCLUSIONS: The different motilities and behaviors of T cells observed using our imaging approach after stroke could reveal important mechanisms of immune surveillance for future therapeutic exploitations.

Author(s):Fumagalli, Stefano, Coles, Jonathan A, Ejlerskov, Patrick, Ortolano, Fabrizio, Bushell, Trevor J, Brewer, James M, De Simoni, Maria-Grazia, Dever, Gary, Garside, Paul, Maffia, Pasquale and Carswell, Hilary V
Item type:Article
ID code:31808
Keywords:Animals, Brain, Disease Models, Animal, Green Fluorescent Proteins, Infarction, Middle Cerebral Artery, Male, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Microscopy, Fluorescence, Multiphoton, Stroke, T-Lymphocytes, Cardiology and Cardiovascular Medicine, Clinical Neurology, Advanced and Specialised Nursing
Subjects:UNSPECIFIED
Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Pure Administrator
Date deposited:17 Jun 2011 08:58
Last modified:27 Mar 2019 04:12
URI:https://strathprints.strath.ac.uk/id/eprint/31808
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