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Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

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Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polyethylenimine polyplex

Aldawsari, Hibah Mobarak and Sundara Raj, Behin and Edrada-Ebel, Ruangelie and Blatchford, David and Tate, Rothwelle and Tetley, Laurence and Dufès, Christine (2011) Enhanced gene expression in tumors after intravenous administration of arginine-, lysine- and leucine-bearing polyethylenimine polyplex. Nanomedicine: Nanotechnology, Biology and Medicine, 7 (5). pp. 815-823.

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Abstract

The potential of gene therapy to treat cancer is currently limited by the low expression of therapeutic genes in the tumors. As amino acids are known to have excellent properties in cell penetration and gene expression regulation, we investigated if the conjugation of arginine, lysine and leucine onto the surface of the gene delivery system polyethylenimine could lead to an improved gene expression in tumors. The intravenous administration of arginine-, lysine- and Leucine-bearing polyethylenimine polyplexes led to a significant increase of gene expression in the tumor, with a β-galactosidase expression amount at least 3-fold higher than that obtained after treatment with unmodified polyethylenimine polyplex. The three amino acid-bearing polyethylenimine led to similar levels of gene expression in the tumor. The treatments were well tolerated by the mice. Arginine-, lysine- and leucine-bearing polyethylenimine are therefore highly promising gene delivery systems for cancer therapy.