Characterisation of P2X receptors expressed in rat pulmonary arteries

Syed, Nawazish-I-Husain and Tengah, Asrin and Paul, Andrew and Kennedy, Charles (2010) Characterisation of P2X receptors expressed in rat pulmonary arteries. European Journal of Pharmacology, 649 (1-3). pp. 342-348. ISSN 0014-2999 (https://doi.org/10.1016/j.ejphar.2010.09.041)

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Abstract

Previous studies indicated that a P2X receptor other than the P2X1 subtype might be present in rat large but not small pulmonary arteries The aim here was to characterise further these P2X receptors Isometric tension was recorded from rat Isolated small (i d 250-500 mu m) and large pulmonary artery (i d 1-1 5 mm) rings mounted on a wire myograph In both tissues the P2X receptor agonist alpha beta meATP evoked rapidly-developing contractions that were inhibited by the P2X antagonists NF449 PPADS and suramin in a concentration-dependent manner and eventually abolished by each The rank order of the potency in both tissues was NF449 > PPADS = suramin For each antagonist there was no significant difference between its potency in the small and large pulmonary arteries Prolonged administration of a high concentration of alpha beta-meATP induced complete desensitisation in both tissues RT-PCR followed by PCR with specific oligonucleotide primers identified mRNA for all seven P2X subunits Subtype-specific antibodies showed strong punctate P2X1 receptor like immunoreactivity in the majority of cells and faint punctate staining with the anti-P2X2 and anti-P2X4 antibodies whilst P2X5-like immunoreactivity was barely detectable and no P2X3 P2X6 and P2X7 receptor-like immunoreactivity was seen No differences in P2X mRNA and protein expression were seen between small and large pulmonary arteries In conclusion the pharmacological properties and mRNA and protein expression profiles of P2X receptors in rat small and large pulmonary arteries are very similar Thus P2X1 appears to be the predominant P2X subunit functionally expressed in smooth muscle cells of rat small and large pulmonary arteries (C) 2010 Elsevier B V All rights reserved

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