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Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

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Glucose sensing based on the intrinsic fluorescence of sol-gel immobilized yeast hexokinase

Hussain, F. and Birch, David J.S. and Pickup, John C. (2005) Glucose sensing based on the intrinsic fluorescence of sol-gel immobilized yeast hexokinase. Analytical Biochemistry, 339 (1). pp. 137-143. ISSN 0003-2697

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Abstract

In this study, we investigated measurements of the intrinsic fluorescence of yeast hexokinase as an assay for glucose and immobilization of the enzyme in a silica sol-get matrix as a potential in vivo glucose sensor for use in patients with diabetes. The intrinsic fluorescence of hexokinase in solution (excitation = 295 nm, emission = 330 nm) decreased by 23% at a saturating glucose concentration of 1 mM (K-d = 0.3 mM), but serum abolished the glucose-related fluorescence response. When entrapped in tetramethylorthosilicate-derived sol gel, hexokinase retained activity, with a 25% maximal glucose-related decrease in intrinsic fluorescence, and the saturation point was increased to 50 mM glucose (K-d = 12.5 mM). The glucose response range was increased further (to 120 mM, K-d = 57 mM) by a covering membrane of poly(2-hydroxyethyl) methacrylate. Unlike free enzyme, the fluorescence responses to glucose with sol-gel immobilized hexokinase, with or Without covering membrane, were similar for buffer and serum. We conclude that fluorescence monitoring of sol-gel entrapped yeast hexokinase is a Suitable system for development as an in vivo glucose biosensor. (c) 2005 Elsevier Inc. All rights reserved.