An air-exposure technique for the formation of artificial lipid bilayers in microsystems
Sandison, Mairi E. and Zagnoni, Michele and Morgan, Hywel (2007) An air-exposure technique for the formation of artificial lipid bilayers in microsystems. Langmuir, 23. pp. 8277-8284. ISSN 0743-7463 (https://doi.org/10.1021/la7007528)
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To develop a reliable method for on-chip bilayer lipid membrane (BLM) formation, which could be employed for use in a biosensor array platform, a polymer microfluidic device has been constructed, and the formation of suspended BLMs within it has been investigated. A simple, yet reproducible BLM formation protocol has been developed, in which a brief air-exposure period is employed to induce the rapid thinning of an initially thick lipid-solvent layer. The technique is rapid, reproducible, and amenable to the simple injection of proteins or analytes, as well as to buffer exchange on both sides of the membrane. Scaling up the technique for use in an array platform is also straightforward, the simultaneous formation of three individually addressable BLMs being demonstrated.
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Item type: Article ID code: 29055 Dates: DateEvent2007PublishedSubjects: Technology > Electrical engineering. Electronics Nuclear engineering Department: Faculty of Engineering > Electronic and Electrical Engineering
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Engineering > Biomedical EngineeringDepositing user: Pure Administrator Date deposited: 22 Mar 2011 12:32 Last modified: 08 Apr 2024 18:49 URI: https://strathprints.strath.ac.uk/id/eprint/29055