Microfluidic array platform for simultaneous lipid bilayer membrane formation

Zagnoni, Michele and Sandison, Mairi E. and Morgan, Hywel (2009) Microfluidic array platform for simultaneous lipid bilayer membrane formation. Biosensors and Bioelectronics, 24 (5). 1235–1240. ISSN 0956-5663 (https://doi.org/10.1016/j.bios.2008.07.022)

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Abstract

In recent years, protein array technologies have found widespread applications in proteomics. However, new methods for high-throughput analysis of protein–protein and protein–compound interactions are still required. In this paper, an array of lipid bilayer membranes formed within a microfluidic system with integrated electrodes is presented. The system is comprised of three layers that are clamped together, thus rendering the device cleanable and reusable. The device microfluidics enable the simultaneous formation of an array of lipid bilayers using a previously developed air-exposure technique, thereby avoiding the need to manually form individual bilayers. The Ag/AgCl electrodes allow for ion channel measurements, each of the sites being independently addressable. Typically, a 50% yield in simultaneous lipid bilayers formation over 12 sites was obtained and ion channel recordings have been acquired over multiple sites. This system has great potential for the development of an automatable platform of suspended lipid bilayers arrays.

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