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Isolation and structure identification of new alkaloids from the sponge Rhabdastrella rowi

Hertiani, T. and Edrada-Ebel, R.A. and van Soest, R.W.M. and Sudarsono, S. and Proksch, P. (2008) Isolation and structure identification of new alkaloids from the sponge Rhabdastrella rowi. Majalah Farmasi Indonesia, 19 (3). pp. 128-136. ISSN 0126-1037

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Abstract

Has conducted research on chemical components Rhabdastrella rowi sea sponges were collected from Bali, Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi struktur metabolit sekunder spons tersebut dan menguji aktivitas sitotoksisnya terhadap kultur sel limfoma tikus L5178Y. This study aimed to isolate and identify the structure of the secondary metabolites of sponges and test sitotoksisnya activity against L5178Y mouse lymphoma cell culture. Isolasi dilakukan dengan menggunakan berbagai tehnik kromatografi. Isolation was done by using various chromatographic techniques. Metode spektroskopi resonansi magnetic inti serta spektrometri massa digunakan untuk mengidentifikasi struktur kimia isolat. Method of magnetic resonance spectroscopy and mass spectrometry core is used to identify the chemical structure of isolates. Pengujian sitotoksisitas pada kultur sel limfoma tikus L5178Y dilakukan dengan menggunakan MTT assay. Cytotoxicity test on L5178Y mouse lymphoma cell culture by using MTT assay.