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Reversible, fluorescence-based optical sensor for hydrogen peroxide

Mills, A. and Tommons, Cheryl and Bailey, R.T. and Tedford, M.Catriona and Crilly, Peter J. (2007) Reversible, fluorescence-based optical sensor for hydrogen peroxide. Analyst, 132 (6). pp. 566-571. ISSN 0003-2654

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The preparation and characterisation are described of a robust, reversible, hydrogen peroxide optical sensor, based on the fluorescent quenching of the dye ion-pair [Ru(bpy)32+(Ph4B-)2], by O2 produced by the catalytic breakdown of H2O2, utilizing the inorganic catalyst RuO2·xH2O. The main feature of this system is the one-pot formulation of a coating ink that, when dried, forms an active single-layer fluorescence-based H2O2 sensor, demonstrably capable of detecting H2O2 over the range of 0.01 to 1 M, with a relative standard deviation of ca. 4% and a calculated lower limit of detection of 0.1 mM. These sensors are sterilisable, using dry-heat, and stable when stored over 40 days, without exhibiting any loss in sensitivity or response characteristics.