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Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

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Quantification of tetracycline resistance genes in feedlot lagoons by real-time PCR

Smith, M.S. and Yang, R.K. and Knapp, C.W. and Niu, Y.F. and Peak, N. and Hanfelt, M.M. and Galland, J.C. and Graham, D.W. (2004) Quantification of tetracycline resistance genes in feedlot lagoons by real-time PCR. Applied and Environmental Microbiology, 70 (12). pp. 7372-7377. ISSN 0099-2240

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A new real-time PCR method is presented that detects and quantifies three tetracycline resistance (Tc-r) genes [tet(O), tet(W), and tet(Q)] in mixed microbial communities resident in feedlot lagoon wastewater. Tc-r gene real-time TaqMan primer-probe sets were developed and optimized to quantify the Tc-r genes present in seven different cattle feedlot lagoons, to validate the method, and to assess whether resistance gene concentrations correlate with free-tetracycline levels in lagoon waters. The method proved to be sensitive across a wide range of gene concentrations and provided consistent and reproducible results from complex lagoon water samples. The log,0 of the sum of the three resistance gene concentrations was correlated with free-tetracycline levels (r(2) = 0.50, P<0.001; n = 18), with the geometric means of individual resistance concentrations ranging from 4- to 8.3-fold greater in lagoon samples with above-median tetracycline levels (>1.95 mug/liter by enzyme-linked immunosorbent assay techniques) than in below-median lagoon samples. Of the three Tc-r genes tested, tet(W) and tet(Q) were more commonly found in lagoon water samples. Successful development of this real-time PCR assay will permit other studies quantifying Tc-r gene numbers in environmental and other samples.