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Driving innovations in manufacturing: Open Access research from DMEM

Strathprints makes available Open Access scholarly outputs by Strathclyde's Department of Design, Manufacture & Engineering Management (DMEM).

Centred on the vision of 'Delivering Total Engineering', DMEM is a centre for excellence in the processes, systems and technologies needed to support and enable engineering from concept to remanufacture. From user-centred design to sustainable design, from manufacturing operations to remanufacturing, from advanced materials research to systems engineering.

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Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms

Gordan, D.A. and Toth, G.P. and Graham, D.W. and Lazorchak, J.A. and Redd, T.V. and Knapp, Charles W. and deNoyelles, F. and Campbell, Scott and Lattier, D.L. (2006) Effects of eutrophication on vitellogenin gene expression in male fathead minnows (pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms. Environmental Pollution, 142 (3). pp. 559-566. ISSN 0269-7491

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Abstract

This study evaluated the effect of aquatic secondary nutrient supply levels (nitrogen and phosphorus) on the subcellular response of adult male fathead minnows (Pimephales promelas) exposed to a single nominal concentration of 17 alpha-ethynytestradiol (EE2), a potent synthetic estrogen, under quasi-natural field conditions. Outdoor mesocosms were maintained under low, medium, and high nutrient supply conditions as categorized by total phosphorus (TP) level (nominal 0.012, 0.025, and 0.045 mg TP/L, respectively), and treated with EE2 with and without a carrier solvent. Using reverse transcription-polymerase chain reaction methods, vitellogenin gene (Vg) expression was determined in the fish collected at 0 h, 8 h, 24 h, 4 d, 7 d, and 14 d post-exposure. Induction of Vg was detected as early as 8 It post-exposure, with and without the carrier solvent, and persisted through Day 14. Results showed Vg to be significantly greater at low nutrient levels (p < 0.05), suggesting that EE2 bioavailability to the fish was likely greater under less-turbid water conditions. Published by Elsevier Ltd.